Journal: OncoTargets and Therapy

Article Title:

Implications of the Receptor Tyrosine Kinase Axl in Gastric Cancer Progression

doi: 10.2147/ott.s257606

Figure Lengend Snippet: Figure 2 Axl overexpression induces EMTand promotes the invasion and proliferation of GC cells. BGC-823 and SGC-7901 cells were transfected with Axl overexpressing plasmid. (A) The expression of Axl mRNA in GC cells was determined RT-PCR assay. (B) The expression of Axl, E-cadherin, and vimentin in GC cells were measured by Western blot assay. Representative blots are shown and GAPDH serve as a loading control. (C) The mRNA expression of E-cadherin (CDH1) and vimentin (VIM) expression levels were examined by RT-PCR assay. (D) Transwell invasion assay was used to detect cell invasion capacities. Representative images and quantification of invaded cells are shown. Scar bar, 200 μm. (E) EdU cell proliferation assay and (F) CCK-8 assay were performed to examine GC cell proliferation. Representative images and quantification of EdU-positive cells are shown. Scar bar, 100 μm. Data are shown as mean ± SEM, n = 3. **P < 0.01 and ***P < 0.001 compared with the vector groups.

Article Snippet: Antibodies against Gas6, Axl, p-Axl (Y702), E-cadherin, vimentin, ZEB1 and GAPDH were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Transwell Invasion Assay, Proliferation Assay, CCK-8 Assay

Journal: OncoTargets and Therapy

Article Title:

Implications of the Receptor Tyrosine Kinase Axl in Gastric Cancer Progression

doi: 10.2147/ott.s257606

Figure Lengend Snippet: Figure 3 Silencing Axl inhibits EMT and suppresses the invasion and proliferation of GC cells. BGC-823 and SGC-7901 cells were transfected with Axl specific siRNA. (A) The expression of Axl in cells were determined by RT-PCR assay. (B) The expression of Axl, E-cadherin, and vimentin in GC cells were detected by Western blot assay. Representative blots are shown and GAPDH serve as a loading control. (C) RT-PCR assay for the expression of CDH1 and VIM expression levels in GC cells. (D) The invasion of GC cells was detected by Transwell invasion assay. Representative images and quantification of invaded cells are shown. Scar bar, 200 μm. (E and F) The proliferation of GC cells was examined by (E) EdU cell proliferation assay and (F) CCK-8 assay. Scar bar, 100 μm. Representative images and quantification of EdU-positive cells are shown. Data are shown as mean ± SEM, n = 3. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the NC siRNA groups.

Article Snippet: Antibodies against Gas6, Axl, p-Axl (Y702), E-cadherin, vimentin, ZEB1 and GAPDH were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Transfection, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Transwell Invasion Assay, Proliferation Assay, CCK-8 Assay

Journal: OncoTargets and Therapy

Article Title:

Implications of the Receptor Tyrosine Kinase Axl in Gastric Cancer Progression

doi: 10.2147/ott.s257606

Figure Lengend Snippet: Figure 5 The knockdown of ZEB1 impairs EMT and blocks the invasion and proliferation in GC cells. BGC-823 and SGC7901 cells were transfected with ZEB1 specific siRNA. (A) RT-PCR assay was used to examine ZEB1 level in GC cells. (B) The expression of ZEB1, E-cadherin and vimentin in GC cells were detected by Western blot assay. Representative blots are shown and GAPDH serve as a loading control. (C) CDH1 and VIM expression levels in GC cells were determined by RT-PCR assay. (D) Transwell invasion assay was performed to detect GC cell invasion. Representative images and quantification of invaded cells are shown. Scar bar, 200 μm. (E) Cell proliferation was examined by EdU cell proliferation assay. Representative images and quantification of EdU-positive cells are shown. Scar bar, 100 μm. Data are shown as mean ± SEM, n = 3. **P < 0.01, and ***P < 0.001 compared with the NC siRNA groups.

Article Snippet: Antibodies against Gas6, Axl, p-Axl (Y702), E-cadherin, vimentin, ZEB1 and GAPDH were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Knockdown, Transfection, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Control, Transwell Invasion Assay, Proliferation Assay